A7058

Sigma

 

Monoclonal Anti-polyHistidine−Peroxidase antibody produced in mouse

clone HIS-1, purified immunoglobulin, lyophilized powder

Synonym:Monoclonal Anti-polyHistidine

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Description

Immunogenrecombinant polyHistidine tagged fusion protein.
Preparation NotePrepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).
Legal InformationThis Product is covered by patent DE 19507166 and foreign equivalents exclusively licensed to QIAGEN GmbH, Qiagen Strasse 1, D-40724 Hilden, Germany.
ReconstitutionThe antibody conjugate should be reconstituted with 0.5 ml deionized water.
SpecificityThe antibody recognizes synthetic polyHistidine, as well as native or denatured, reduced forms of proteins tagged with 6X histidines, expressed in selected vectors.
Physical formLyophilized from 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 0.01% thimerosal

Properties

antibody formpurified immunoglobulin
cloneHIS-1 (monoclonal)
formlyophilized powder
packagingvial of 0.5 mL
application(s)immunoblotting: 1:2,000 using lysates of Escherichia coli induced to express a 6xHis tagged protein
isotypeIgG2a
storage temp.2-8°C

Safety

WGK Germany3